cancer cell lines human prostate pc3  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the proliferation of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mLof PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added and cell proliferation was monitored by analyzing the surface area occupied by the cells in each well (% confluence) from photos taken at regular intervals (every 3 h) by the Incucyte live cell imaging system over a period of three days. Data shown are the average ± SD of three independent experiments.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, Live Cell Imaging

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the viability of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mL of PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added. 24h later, cell viability was assessed using the MTT assay. Data shown are the average ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, MTT Assay, Comparison

Journal: ACS Omega

Article Title: Novel C-2 Aromatic Heterocycle-Substituted Triterpenoids Inhibit Hedgehog Signaling in GLI1 Overexpression Cancer Cells

doi: 10.1021/acsomega.4c11479

Figure Lengend Snippet: Effect of compounds 11a and 11b on the protein expression of the Gli1 transcription factor and its transcriptional targets in A549 (A) and DU145 (B) cells following 24 h of incubation. An antibody against GAPDH was used as an internal loading control.

Article Snippet: The human prostate tumor-derived cell line DU145 was obtained from ATCC (American Type Culture Collection) and cultured in RPMI 1640 (Roswell Park Memorial Institute) medium supplemented with 10% (v/v) fetal bovine serum (FBS).

Techniques: Expressing, Incubation, Control

Journal: Scientific Reports

Article Title: Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression

doi: 10.1038/s41598-024-72408-w

Figure Lengend Snippet: Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.

Article Snippet: Androgen-dependent 22RV1, LNCAP, VCAP, C4-2 and androgen-independent DU145and PC3 human tumor prostate cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Over Expression, Western Blot